Possibly. Many moms find that if they don't pump/nurse frequently, their output drops during the hours when they cannot remove milk. The good news is that your body has a terrific internal clock, and even if you experience a supply decrease during the workday, your body will know to make enough milk for your baby's needs when you're with him.
Well, my question is since I am able to pump only once during the day would my milk supply become less over time?
This is a hard one to answer, since the answer would be different for every mom. You sort of have to tinker with your routine and see what works for you. If what you're doing isn't working, then it's time to try something else.
I have also noticed that whether I pump twice in a day or once after a longer duration the output remains more or less the same. Can I continue in this manner or should I be doing something different?
Coolest thing my big girl said recently: "How can you tell the world is moving when you are standing on it?"
Coolest thing my little girl sang recently: "I love dat one-two pupples!"